卵泡发育相关基因在牛优势和从属卵泡颗粒细胞中表达的研究

doi:10.11843/j.issn.0366-6964.2014.12.007

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (12): 1957-1963.doi: 10.11843/j.issn.0366-6964.2014.12.007

卵泡发育相关基因在牛优势和从属卵泡颗粒细胞中表达的研究

姚晓磊¹，李鹏飞²，姜晓龙¹，孟金柱¹，黄洋¹，刘岩¹，陈建伟¹，赵妙妙¹，吕丽华^1*

（1.山西农业大学动物科技学院，太谷 030801； 2.山西农业大学生命科学学院，太谷 030801)

收稿日期:2014-05-22 出版日期:2014-12-23 发布日期:2014-12-23
通讯作者: 吕丽华， E-mail：sxaullh@yahoo.com.cn
作者简介:姚晓磊（1989-)，男，山西运城人，硕士生，主要从事动物遗传育种与繁殖研究，E-mail：shitoukuai_05@163.com
基金资助:
国家自然科学基金（31172211）；农业部948项目（2010-Z43）；山西省国际科技合作项目(2010081002)；山西农业大学新增横向协作与委托项目（2010HX54）；国家重点实验室开放课题 (SNKF-2012-02)；山西省科技攻关项目（20130311027-2）；山西省回国留学人员科研资助项目（2014-重点5）；山西农业大学引进人才博士科研启动费(2014ZZ04)

Research of the Genes Expression Associated with Follicular Development in Bovine Dominant and Subordinate Follicle Granulose Cells

YAO Xiao-lei¹,LI Peng-fei²，JIANG Xiao-long¹，MENG Jin-zhu¹,HUANG Yang¹，LIU Yan¹，CHEN Jian-wei¹,ZHAO Miao-miao¹,LYU Li-hua^1*

（1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China;2.College of Life Science，Shanxi Agricultural University，Taigu 030801,China）

Received:2014-05-22 Online:2014-12-23 Published:2014-12-23
Supported by:

摘要/Abstract

摘要：

旨在探究卵泡发育相关基因在牛发情周期内第一卵泡波中优势卵泡（Dominant follicles，DF）和从属卵泡（Subordinate follicles，SF）颗粒细胞中表达差异，进而筛选影响牛卵泡发育的相关基因。通过GEO数据库与基因功能分析筛选与牛卵泡发育相关基因，采用qRT-PCR检测牛第一卵泡波DF和SF颗粒细胞中各基因的表达差异。结果显示：筛选出6个候选基因（SFRP2、CPEB1、PRKAG2、MAPK8、PPP2R2A和PRSS23）与牛卵泡发育有关；qRT-PCR检测，CPEB1在DF中的表达量极显著高于SF（P＜0.01），PRKAG2在DF中的表达量显著高于SF（P＜0.05）；SFRP2和MAPK8在SF中的表达量极显著高于DF（P＜0.01）；PPP2R2A和PRSS23虽然在DF和SF的表达量存在倍数关系，但差异并不显著。综上表明，在牛卵泡发育过程中，CPEB1和PRKAG2促进卵泡发育，SFRP2和MAPK8对卵泡的发育可能有抑制作用。

Abstract:

To study the regulatory mechanism of the relevant genes associated with follicular development，we test the genes expression difference between the dominant follicles (DF) and subordinate follicles (SF) granulose cells of the first follicular wave during estrous cycle in bovine.Bioinformatics methods were used to screen the candidate genes associated with follicular development，and real-time fluorescent quantitative PCR was performed to detect the gene expression pattern between DF and SF granulose cells of the first follicular wave during estrous cycle in bovine.The results showed that 6 candidate genes (SFRP2，CPEB1，PRKAG2，MAPK8,PPP2R2A and PRSS23) related to follicular development were screened.CPEB1 expressed in DF was extremely significantly higher than that in SF (P<0.01)，and PRKAG2 mRNA amounts were significantly greater in DF compared with SF (P<0.05).But the expression levels of SFRP2 and MAPK8 were extremely significantly higher in SF than those in DF (P<0.01).CPEB1 and PRKAG2 may play a crucial role to promote the follicular development，however，SFRP2 and MAPK8 may inhibit the follicular development in bovine.

中图分类号:

S823.2

姚晓磊，李鹏飞，姜晓龙，孟金柱，黄洋，刘岩，陈建伟，赵妙妙，吕丽华. 卵泡发育相关基因在牛优势和从属卵泡颗粒细胞中表达的研究[J]. 畜牧兽医学报, 2014, 45(12): 1957-1963.

YAO Xiao-lei,LI Peng-fei，JIANG Xiao-long，MENG Jin-zhu,HUANG Yang，Lü Yan，CHEN Jian-wei,ZHAO Miao-miao,LYU Li-hua. Research of the Genes Expression Associated with Follicular Development in Bovine Dominant and Subordinate Follicle Granulose Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1957-1963.

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卵泡发育相关基因在牛优势和从属卵泡颗粒细胞中表达的研究

Research of the Genes Expression Associated with Follicular Development in Bovine Dominant and Subordinate Follicle Granulose Cells

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